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Alkaline Lysis Buffer A Recip

       Add the following to 100ml distilled H2O

       5ml of 20% SDS

       2ml of 10M NaOH


Alkaline Lysis Buffer B Recipe

       Add the following to 100ml distilled H2O

29.4g KAc

11.5ml of Glacial Acetic Acid


Alkaline Lysis Buffer C Recipe

Add the following to 900ml distilled H2O

9g Glucose

3g Tris

20ml of 0.25M EDTA


q.s. to 1000ml with distilled H2O

Autoclave using standard conditions

Allow to cool to room temperature

Store at 4℃


When ready to use add 0.02g lysozyme to 100ml of alkaline lysis buffer C to yield alkaline lysis buffer A.