PCNA Mouse mAb
- 应用: WB,Flow,ICC/IF
- 种属反应性: Human,Mouse ,Rat
- 亚型: IgG1
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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
Ab155826-20μl | 20μl | 期货 |
|
|
Ab155826-50μl | 50μL |
现货
|
|
|
Ab155826-100μl | 100μL | 现货 |
|
|
Ab155826-1ml | 1ml | 期货 |
|
图片
PCNA Mouse mAb (Ab155826) - Western Blot
All lanes:
PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Samples:
Lysates at 20 µg per lane
Secondary:
Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size:
29 kDa
Observed band size:
29 kDa
PCNA Mouse mAb (Ab155826) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Ab155826) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
PCNA Mouse mAb (Ab155826) - Flow Cytometry
Flow analysis of PCNA (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. PCNA Mouse mAb (Ab155826) at 1/4000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
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